Figure 2.

ADF1 encodes a TRP family ion channel, TRP15. (A) Schematic diagram of TRP15, showing mutation sites in indicated alleles along with the PFAM ion channel prediction. (B) Deflagellation phenotypes of the adf1 alleles. Each value is the mean of two or more independent experiments ± SEM. (C) PCR confirms the presence of a deletion in TRP15 in adf1-3, which is restored in adf1-3R. Of the TRP15 primers, one anneals within the deleted sequence and the other anneals just upstream of it. IFT20 was PCR-amplified in the same reaction as a positive control. (D) Phylogenetic analysis placing TRP15 in the context of metazoan TRP clades.