Figure 5.

Effects of selection strength, incubation time, and significance threshold on interactions observed between TFs and yellow fragments. The number of TFs with a significant interaction with at least one yellow enhancer fragment (y-axis) at a given significance threshold or smaller [x-axis, –log(p-value)] is shown. The different colored dots show results using plates with different levels of inhibitor (10 mM, 20 mM, 40 mM of 3AT), two different incubation times (7 or 10 d of growth allowed), and when we required evidence of an interaction with at least one, two, or three levels of 3AT inhibitor, as indicated in the figure inset. Note that the rate of decrease in the number of TFs that showed a significant interaction with at least one yellow enhancer fragment slowed down around –log(p-value) = 2, which corresponds to p-value ≤ 0.01. Results discussed in the manuscript were based on a statistical threshold of p-value ≤ 0.005, or –log(p-value) = 2.3 and the requirement that a given TF-enhancer fragment interaction was observed with at least two 3AT levels.