Figure 7.

Summary of RNAi knockdown phenotypes observed for 32 transcription factors. Schematic representations of abdominal pigmentation in wild type D. melanogaster (A), and after knocking down activity of 32 transcription factors (B–E) are shown. Four classifications were used to describe the pigmentation phenotypes of RNAi knockdown flies: reduced dark pigment in female segment A6 only (B), reduced dark pigment in A5 and/or A6 in one or both sexes (C), reduced or lost dark pigment in multiple segments in one or both sexes (D), and increased dark pigment in stripes and/or midline peak in one or both sexes (E). Knockdown phenotypes of 21 transcription factors each fell into only one of these four categories, whereas knockdown phenotypes of 11 transcription factors were more complex and fell into more than one category.