Figure 8.

RNAi knockdown phenotypes for nuclear receptor genes showing evidence of binding to yellow fragments in yeast-one-hybrid screen. Abdominal pigmentation phenotypes in both control and knockdown flies of both sexes are shown for four nuclear receptors, Hr78, Hr38, Hr46, and Eip78C. Changes in pigmentation observed consistently in knockdown relative to control flies are marked with arrows and described further in Table S4. Briefly, knockdown of Hr78, Hr46, and Eip78C resulted in increased dark pigment in female segments A3, A4, A5, and male segments A3 and A4 as well as reduced dark pigment in female segment A6. Knockdown of Hr38 only led to reduced dark pigment in female segment A6.