Figure 2.

An RNAi screen for suppressors of the embryonic lethality of the cul-2(or209ts) temperature-sensitive allele. (A) Schematic representation of the cul-2(or209ts) RNAi-based suppressor screen. Synchronized cul-2(or209ts) L1 larvae were fed in 24-well plates with bacteria expressing dsRNA at 15° until the L4/young adult stage, then the plates were placed at the semipermissive temperature (23°). After 2 d, the wells containing larvae, as opposed to dead embryos, were selected as positive (green wells). The black well contained a negative (ctrl) and the blue well a positive (atl-1) control. (B) Graphs showing the percentage of viability of cul-2(or209ts) animals after RNAi-mediated depletion of the indicated genes. Each dot on the graph corresponds to an independent experiment. Numerical values and statistical analysis are provided in Table S2. (C) Genes identified in the cul-2(or209ts) (purple circle), lrr-1(0) suppressor screens (blue circle), and in both screens.