Conditional ADAM17 knockout (KO) and control mice were subjected to CLP and monitored for 5 d for survival. (A) 18-G, 2-puncture CLP; n = 9 mice per group; P < 0.002, log-rank test. (B) Conditional ADAM17 knockout and control mice were subjected to CLP (18-G, 2-puncture), and sepsis severity was assessed at 12 h using a murine sepsis scoring system to evaluate various physiologic variables, as described in Materials and Methods; n = 9 mice per group; ***P < 0.001. (C) 21-G, 1-puncture CLP; n = 11 mice per group; P < 0.0001, log-rank test.