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. 2016 Apr 8;100(5):999–1004. doi: 10.1189/jlb.3VMAB1115-496RR

Figure 4. ADAM17 deficiency in leukocytes increases neutrophil recruitment during sepsis.

Figure 4.

(A) High-throughput DNA sequencing of the V5–V6 hypervariable regions of the 16S rRNA gene was performed on DNA samples isolated from the cecal contents of unmanipulated, conditional ADAM17 knockout and control mice. Taxa distribution (Observed OTUs) and richness (Chao1) within cecal bacterial communities from conditional ADAM17 knockout and control mice (left), as well as their overlap (right) were determined; n = 4 mice per group. ns, Not significant; PC1/2, principal coordinates 1/2. (B) qPCR of the 16S rRNA gene in the cecal contents of unmanipulated conditional ADAM17 knockout and control mice; n = 4 mice per group. (C) Conditional ADAM17 knockout and control mice were subjected to CLP (18-G, 2-puncture). Peritoneal lavage was performed at the indicated time points after CLP and neutrophil counts determined: 12 h post-CLP, n = 6 mice per group; 15 h post-CLP, n = 4 mice per group; 24 h post-CLP, n = 9 mice per group.