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. 2016 Jun 16;100(5):1113–1124. doi: 10.1189/jlb.3A1015-463R

Figure 6. Rescue of lipotoxic cell death by mTOR inhibition occurs independent of autophagy.

Figure 6.

(A) WT and ATG5KO pMACs were treated with BSA-PBS or palm-LPS ± torin, and cell death was assessed by annexin-PI flow cytometry. (B) WT and ATG16LKO pMACs were treated with BSA-PBS or palm-LPS ± torin, and cell death was assessed by annexin-PI flow cytometry. (C) WT and p62 KO pMACs were treated with BSA-PBS or palm-LPS ± torin, and cell death was assessed by annexin-PI flow cytometry. For each KO mouse, total protein was isolated and analyzed for p62 ± LC3 by Western blotting. veh, vehicle. Bar graphs report means ± se for a minimum of 3 experiments, each performed in triplicate. *P < 0.05 for BSA-PBS vs. palm-LPS; #P < 0.05 for veh vs. torin.