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. 2004 Sep;78(17):9123–9131. doi: 10.1128/JVI.78.17.9123-9131.2004

FIG. 4.

FIG. 4.

Function of MHV-68 oriLyt during viral reactivation. (A) pMOΔ2 or pKS and an RTA-expressing plasmid, pFlag/MRTA, or pFlag-CMV-2, were cotransfected by electroporation into S11E cells, a murine B-cell line harboring latent MHV-68. At 24 h posttransfection, DNA was prepared from each sample and probed with radiolabeled pKS for Southern blot analysis. Expression of MHV-68 RTA induced latent MHV-68 in S11E cells to reactivate and thus the oriLyt plasmid pMOΔ2 to replicate. (B) The oriLyt deletion constructs pMOΔ5, pMOΔ12, and pMOΔ16 were tested in S11E cells as described for A. Lower bracket, DpnI-sensitive input DNA. Upper bracket, DpnI-resistant replicated DNA.