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. 2004 Sep;78(17):9243–9256. doi: 10.1128/JVI.78.17.9243-9256.2004

FIG. 6.

FIG. 6.

Expression and purification of recombinant wt 2C and deletion mutants. (A) Diagram of wt and various 2C deletion mutants; (B) V5-tagged wt 2C or various 2C mutants were expressed in E. coli and purified by affinity chromatography. The figure shows a Western blot of wt 2C (lane 2) and deletion mutants NΔ35 (lane 3), NΔ70 (lane 4), NΔ105 (lane 5), Δ101-270 (lane 6), CΔ35 (lane 7), CΔ70 (lane 8), or CΔ105 (lane 9) with anti-V5 tag antibody. In lane 1, proteins purified thorough an affinity column from bacterial extracts without the 2C plasmid were analyzed.