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. Author manuscript; available in PMC: 2017 Mar 12.
Published in final edited form as: Nat Chem Biol. 2016 Sep 12;12(11):937–943. doi: 10.1038/nchembio.2172

Figure 2.

Figure 2

HeLa and H460 cells cultured in 13C3-lactate have labeled TCA cycle intermediates. The patterns shown represent the isotopologue distribution of citrate, α-ketoglutarate, and malate as measured by LC/MS. Data are from (a) HeLa cells or (b) H460 lung cancer cells cultured for 3 hours in standard media (containing natural-abundance glucose) that was supplemented with either 3 mM 13C3-lactate (red) or natural-abundance lactate (black). Data shown are mean values ± s.d. (n = 3).