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. Author manuscript; available in PMC: 2017 Mar 19.
Published in final edited form as: Nat Chem Biol. 2016 Sep 19;12(11):905–907. doi: 10.1038/nchembio.2187

Figure 1.

Figure 1

C5′-Extension in antifungal PN biosynthesis. a. Proposed biosynthetic pathway for polyoxins and nikkomycins. The asterisks show the fate of C-3 of PEP. UMP is the only experimentally demonstrated precursor5,9 and the timing of nucleobase modifications is unknown. b. HPLC analysis of PolH assays and negative controls without EP-UMP, SAM, or dithionite, or with heat-inactivated PolH. Also shown is a result of NikJ assay (bottom trace). c. 1H and 2H NMR spectra of U-OAP formed in the PolH assay in deuterium oxide. The H-7′ signal was absent in the 1H NMR spectrum and, instead, observed in the 2H NMR spectrum (compare with Supplementary Fig. 7). The 1H NMR signal with an asterisk at 2.5 ppm is an unidentified impurity.