Figure 3. Recovery of diastolic dysfunction in cRbm20^ΔRRM-raloxifene mice 4 weeks after TAC/DOCA surgery: Pressure-Volume analysis.

DMSO (control) and raloxifene TAC/DOCA cRbm20^ΔRRM mice were studied by PV analysis. A) Representative examples of PV loops. PV analysis was performed on anesthetized and ventilated mice under isoflurane. Inferior vena caval occlusions were conducted to assess load-independent parameters. (B) Diastolic stiffness coefficient β of EDPVR was significantly increased in the cRbm20^ΔRRM-DMSO group but was normal in cRbm20^ΔRRM-raloxifene group. (C) LVEDP was elevated in cRbm20^ΔRRM-DMSO mice. * p≤0.05; **p≤0.01; ****p≤0.0001. (n=12 sham, 21 cRbm20^ΔRRM-DMSO, 28 cRbm20^ΔRRM-raloxifene)