Figure 7. Exercise tolerance study.

(A) Exercise tolerance was evaluated by measuring the running distance in a treadmill running test at three times: preoperative and at 2 weeks and at 4 weeks post TAC/DOCA surgery. The running distance was calculated as a percent of the preoperative running distance of each individual mouse. Sham (circle), cRbm20^ΔRRM-DMSO (upward triangle) and cRbm20^ΔRRM-raloxifene (inverted triangle). (B) At 4 weeks post-surgery (after expression of N2BAsc titin in the raloxifene group), the cRbm20^ΔRRM-raloxifene mice showed a significantly increased running distance compared to cRbm20^ΔRRM-DMSO mice. (For analysis, see Supplemental Methods.) **p≤0.01. (n= 7 sham, 10 cRbm20^ΔRRM-DMSO,10 cRbm20^ΔRRM-raloxifene mice).