Table 1.
Echocardiographic parameters, pressure volume analysis parameters and tissue morphometry at 4 weeks post TAC/DOCA surgery (with expression of N2BAsc titins in the cRbm20ΔRRM-raloxifene group).
| At 4 weeks post- surgery |
Sham |
cRbm20ΔRRM --DMSO |
cRbm20ΔRRM --raloxifene |
|---|---|---|---|
| Age (days) | 85 ± 2 | 84 ± 2 | 84 ± 1 |
| Body Weight, BW (gm) | 23.7 ± 0.6 | 25.7 ± 0.5* | 24.3 ± 0.3# |
| Tibial Length, TL(mm) | 17.0 ± 0.1 | 17.0 ± 0.1 | 17.0 ± 0.1 |
| Echocardiography | |||
| Heart Rate (bpm) | 517 ± 13 | 501 ± 16 | 501 ± 7 |
| LVID;d (mm) | 3.85 ± 0.06 | 4.02 ± 0.06 | 4.15 ± 0.06** |
| WT;d (mm) | 0.80 ± 0.05 | 0.98 ± 0.02** | 0.95 ± 0.02 |
| LVID;s (mm) | 2.72 ± 0.06 | 2.90 ± 0.08 | 3.05 ± 0.08* |
| WT;s (mm) | 1.05 ± 0.06 | 1.29 ± 0.02**** | 1.26 ± 0.02*** |
| Eccentricity | 4.6 ± 0.2 | 4.1 ± 0.1** | 4.4 ± 0.1 |
| EF (%) | 56.8 ± 1.4 | 54.5 ± 1.8 | 52.2 ± 1.5 |
| LA dimension (mm) | 2.13 ± 0.03 | 2.46 ± 0.02**** | 2.43 ± 0.03**** |
| E decel time (ms) | 26.8 ± 0.6 | 20.9 ± 0.5**** | 25.0 ± 0.5#### |
| MV E/A | 1.40 ± 0.06 | 2.46 ± 0.24*** | 1.93 ± 0.16 |
| MV E/é | −36.9 ± 1.7 | −45.3 ± 2.2* | −40.2 ± 1.6 |
| Pressure-Volume Analysis | |||
| Load Dependent Parameters | |||
| ESP (mmHg) | 96 ± 2 | 128 ± 3**** | 128 ± 3**** |
| EDP (mmHg) | 1.6 ± 0.6 | 4.7 ± 0.7** | 2.5 ± 0.4 # |
| dPmax (mmHg/s) | 10,101 ± 426 | 9,089 ± 530 | 9,145 ± 488 |
| dPmin (mmHg/s) | −10,091 ± 567 | −9,870 ± 468 | −10,621 ± 475 |
| ESV (µl) | 24 ± 2 | 39 ± 3** | 41 ± 2** |
| EDV (µl) | 63 ± 4 | 75 ± 3 | 80 ± 3** |
| SV (µl) | 39 ± 2 | 37 ± 1 | 39 ± 2 |
| Tau Glantz (ms) | 8.2 ± 0.5 | 11.5 ± 0.6** | 10.2 ± 0.7 |
| Ea (mmHg/µl) | 2.5 ± 0.1 | 3.7 ± 0.1*** | 3.6 ± 0.2** |
| Load Independent Parameters | |||
| Ees (mmHg/µl) | 4.01 ± 0.38 | 4.49 ± 0.53 | 3.39 ± 0.25 |
| PRSW (mmHg) | 95.3 ± 3.5 | 107.6 ± 7.4 | 105.4 ± 5.3 |
| EDPVR(mmHg/µl) | 0.024 ± 0.002 | 0.059 ± 0.004**** | 0.029 ± 0.003 #### |
| Tissue Morphometry | |||
| LV/TL (mg/mm) | 4.85 ± 0.10 | 7.31 ± 0.32**** | 6.92 ± 0.27*** |
| RV/TL (mg/mm) | 1.23 ± 0.05 | 1.32 ± 0.05 | 1.31 ± 0.04 |
| Lt ATR/TL (mg/mm) | 0.21 ± 0.01 | 0.36 ± 0.03 | 0.37 ± 0.04* |
| Rt ATR/TL (mg/mm) | 0.14 ± 0.02 | 0.23 ± 0.01** | 0.20 ± 0.01* |
| Lung/TL (mg/mm) | 8.55 ± 0.22 | 9.45 ± 0.27* | 9.16 ± 0.17 |
LV, left ventricle; d,diastole; s,systole; LVID, LV internal dimension; WT, wall thickness; Eccentricity indicates LVIDd/WTd; Vol, volume; SV, stroke volume;FS, fractional shortening; EF, ejection fraction; LA, left atrium; MV, mitral valve. ESP: end-systolic pressure; EDP: end-diastolic pressure; dPmax:maximal rate of pressure change; dPmin:minimal rate of pressure change; ESV: end-systolic volume; EDV: end-diastolic volume; SV: stroke volume; EF: ejection fraction; Tau Glantz: LV relaxation time constant ; Ea: effective arterial elastance; Ees: end systolic elastance; PRSW: preload recruitable stroke work; EDPVR: end diastolic pressure volume relation; HW: whole heart weight; RV: right ventricle; ATR: atria. TL: tibial length.
p≤0.05,
p≤0.01,
p≤0.001,
p≤0.0001 vs. Sham;
p≤0.05,
p≤0.0001 vs. cRbm20ΔRRM-DMSO group. (n=10,29,37 mice for echocardiography, n=12,21,28 mice for PV analysis, n=12,22,29 mice for tissue morphometry)