TABLE 1.
Levels of synthesis and stability of Gag and Pol proteinsd
| Virus | Pol
|
Gag
|
Gag/Polc | ||
|---|---|---|---|---|---|
| Expressiona | Stabilityb | Expression | Stability | ||
| IN- | 1.00 | 0.99 | 1.00 | 0.59 | 1.0 |
| GR3st | 0.64 | 1.30 | 1.27 | 0.44 | 2.0 |
| GR2st | 0.15 | 0.95 | 0.63 | 0.62 | 4.2 |
| GR1st | 0.32 | 1.01 | 0.80 | 0.42 | 2.5 |
| GR(-)st | 0.13 | 1.19 | 0.77 | 0.40 | 5.9 |
Expression levels were calculated from the time zero points in Fig. 4, relative to that for the IN- mutant.
Stability levels were calculated by comparing the protein level at 8 h relative to that at 0 h for each mutant.
Ratios were determined from the time zero points.
Results are the averages of three experiments.