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. 2016 Sep 3;53(9):3522–3531. doi: 10.1007/s13197-016-2329-2

Table 2.

Linear regression equations, test ranges, LODs, LOQs, intra and inter-day for quantified compounds

Component Regression equationa r2b Test range (ng/ml) LODc (ng/ml) LOQd (ng/ml) Intra-day (ng/ml) RSDe (%) Inter-day (ng/ml) RSD (%)
Catechin y = 7.5769x − 12.345 0.99 8.93–142.85 1.77 5.31 0.88 0.60
Epicatechin y = 13.675x + 26.547 0.99 4.46–142.85 0.73 2.19 0.61 0.59
Quercetin y = 48.136x − 1.7448 0.99 1.1–17.85 0.29 0.87 0.64 0.65
Kaempferol y = 165.28x − 12.111 0.99 0.55–17.85 0.32 0.96 0.95 0.52
Gallic acid y = 149.52x − 158.06 0.97 1.11–17.85 1.27 3.81 2.30 0.75
P-Coumaric acid y = 73.729x + 0.8202 1.0 0.57–17.85 0.26 0.78 0.74 0.45
Syringic acid y = 75.158x + 2.2644 0.99 2.23–17.85 0.27 0.81 0.56 0.74

aThe regression equation were represented as y = mx + c. y, peak area; x, concentration of compounds (ng/ml)

b r2 is the correlation coefficient of the equation

c LOD, limit of detection, S/N = 3

d LOQ, limit of quantification, S/N = 10

e RSD (%) = (SD/mean) × 100