Table 2.
Component | Regression equationa | r2b | Test range (ng/ml) | LODc (ng/ml) | LOQd (ng/ml) | Intra-day (ng/ml) RSDe (%) | Inter-day (ng/ml) RSD (%) |
---|---|---|---|---|---|---|---|
Catechin | y = 7.5769x − 12.345 | 0.99 | 8.93–142.85 | 1.77 | 5.31 | 0.88 | 0.60 |
Epicatechin | y = 13.675x + 26.547 | 0.99 | 4.46–142.85 | 0.73 | 2.19 | 0.61 | 0.59 |
Quercetin | y = 48.136x − 1.7448 | 0.99 | 1.1–17.85 | 0.29 | 0.87 | 0.64 | 0.65 |
Kaempferol | y = 165.28x − 12.111 | 0.99 | 0.55–17.85 | 0.32 | 0.96 | 0.95 | 0.52 |
Gallic acid | y = 149.52x − 158.06 | 0.97 | 1.11–17.85 | 1.27 | 3.81 | 2.30 | 0.75 |
P-Coumaric acid | y = 73.729x + 0.8202 | 1.0 | 0.57–17.85 | 0.26 | 0.78 | 0.74 | 0.45 |
Syringic acid | y = 75.158x + 2.2644 | 0.99 | 2.23–17.85 | 0.27 | 0.81 | 0.56 | 0.74 |
aThe regression equation were represented as y = mx + c. y, peak area; x, concentration of compounds (ng/ml)
b r2 is the correlation coefficient of the equation
c LOD, limit of detection, S/N = 3
d LOQ, limit of quantification, S/N = 10
e RSD (%) = (SD/mean) × 100