FIG. 4.
Effects of DEN-2 infection on IFN-α-stimulated tyrosine phosphorylation of Stat1. BHK-21 (A) and A549 (B) cells were mock infected or infected with DEN-2 (MOI = 5) for various hours as indicated and then stimulated with IFN-αA/D (1,000 U/ml) for 30 min. Cell lysates were analyzed by Western blotting with anti-phospho-Stat1 (Tyr701) and anti-Stat1 antibodies. The bands intensities were quantified by MetaMorph (Universal Imaging Corp.), and the ratios of pY-Stat1 to Stat1 are shown.