FIG. 8.

Expression of ISGs in JEV-infected cells. (A) A549 cells were mock infected or infected with JEV (MOI = 5) for 6 h and then cultured in the presence or absence of IFN-αA/D (1,000 U/ml) for 16 h. The cell lysates were analyzed by Western blotting with the antibodies indicated on the right side of each panels. (B) The bands intensities were quantified by MetaMorph (Universal Imaging Corp.), and the relative intensities for each protein bands are shown. (C) A549 cells were mock infected or infected with JEV (MOI = 5) for 6 h and then cultured in the presence or absence of IFN-αA/D (1,000 U/ml) for 3 or 6 h. The total cellular RNA was analyzed by RT-PCR as described in Materials and Methods. The expected sizes of PCR fragments for each gene are shown on the right sides of the panels.