Figure 4.

Glycine increases ERK1/2 phosphorylation independent of NMDAR channel activity in hippocampal neurons. (A) Glycine (100 μM) increases p-ERK1/2 after NMDARs and glycine receptors are inhibited (n = 8, ^*p < 0.05). (B) Glycine-induced increase of ERK1/2 phosphorylation is dose-dependent in hippocampal neurons where NMDARs and glycine receptors are inhibited. (C) BAPTA (5.0 mM) treatment does not influence glycine elevation of ERK1/2 phosphorylation in hippocampal neurons where NMDARs and glycine receptors are inhibited (n = 6, ^*p < 0.05). (D) At normal level of extracellular Ca²⁺, glycine (100 μM) increases p-ERK1/2 in hippocampal neurons where NMDARs and glycine receptors are inhibited (n = 6, ^*p < 0.05). Con: Control; Gly: glycine.