Fig. 8.

Effect of e-TRAP, synthetic TRAP, and EMD on the expression of ang-2 in HUVECs. a Relative gene expression level of angiopoietin-2 upon stimulation with e-TRAP, synthetic TRAP, or EMD at concentrations 10–50 μg/ml for 24 h. GAPDH was used as endogenous control gene. ECM supplemented with 0.0005 % acetic acid served as vehicle control (=1). c, b The levels of ang-2 proteins in conditioned media measured by commercially available ELISA. Data are presented as mean ± S.E.M. *P < 0.01, significantly higher compared to vehicle control