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. 2016 Oct 5;2016:8734894. doi: 10.1155/2016/8734894

Figure 2.

Figure 2

Purification of TvoOgg recombinant protein from E. coli. A Coomassie brilliant blue stained SDS-polyacrylamide gel (15%) showing separation of a lysate of BL21 (DE3) cells containing pET28a-TvoOgg after induction by 1 mM IPTG for 5 hr (lane 1), eluted fractions of TvoOgg from Ni-NTA agarose columns (lane 2–9), or SP Sepharose FF columns (lane 10–14), respectively, and TvoOgg protein after dialysis against dialysis buffer 1 (lane 15). The TvoOgg protein is indicated by an arrow. Lane M contained molecular mass standards (Bio-Rad, U.S.A.) as indicated on the left.