TABLE 5.
Binding kinetics of m14 to gp120/gp140 measured by Biacorea
| HIV-1 gp140 |
ka (105 M−1 s−1)
|
kd (10−4 s−1)
|
Kd (nM)
|
|||
|---|---|---|---|---|---|---|
| m14 | b12 | m14 | b12 | m14 | b12 | |
| gp14089.6 (tethered) (clade B) | 3.4 | 2.0 | 5.0 | 4.8 | 1.4 | 2.4 |
| gp140GXC-44 (clade C) | 1.0 | 2.2 | 2.0 | 3.4 | 2.1 | 1.6 |
| gp140R2 (clade B) | 1.2 | 2.4 | 2.3 | 3.2 | 1.9 | 1.3 |
a
The experiments were performed as described in Materials and Methods and in the legend to Fig. 3 but with different Env proteins on the chip. The association (ka) and dissociation (kd), rate constants were calculated by fitting both association and dissociation phases with a 1:1 Langmuir global model, and the equilibrium dissociation constant (Kd) was determined from their ratio.