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. 2016 Oct 5;2016:2407459. doi: 10.1155/2016/2407459

Figure 6.

Figure 6

IgG integrity evaluated by SDS-PAGE. Reduced (using beta-mercaptoethanol) and nonreduced sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used for size-based separations of the IgG for determining IgG integrity in the PTSgel in vitro release rate samples over time. The IgG released from 10GH (a), 103GH (b), and 113GH (c) had the same bands of approximately 150 kDa for the nonreduced and 28 and 51 kDa for the reduced SDS-PAGE at each time point tested, which appeared identical to the IgG standards. D, release day; B, blank lanes; M, molecular size marker; IgG stds., IgG standards. (d) and (e) SE-HPLC analysis of IgG reference standard (d) and of released sample (e) after incubation with PTSgel 10GH for 28 days in PBS at 37°C.