FIG. 1.

3′ UTR deletion mutants of an infectious cDNA clone of GBV-B (pGBB) (4). Deleted sequences are shown in black boxes. The 3′ UTR of GBV-B has 362 nt consisting of the ORF stop codon, a short proximal sequence of 27 nt (V region), a poly(U) tract of 23 nt, and a 3′-terminal sequence of 309 nt (3′X region). In the pGBB-GFP construct, EMCV IRES and GFP sequences were inserted into the short proximal sequence of the 3′ UTR. The genome sequence recovered from an animal transfected with pGBB-GFP is indicated. Note that in this sequence a portion of the V region and the poly(U) region were deleted, but 68 nt of the original IRES-GFP insert were retained.