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. 2016 Sep 15;12(10):1225–1235. doi: 10.7150/ijbs.15154

Figure 3.

Figure 3

Deficiency of DJ-1 attenuates liver damage and ROS generation in CCl4-induced acute liver injury. (A) HE staining and serum ALT levels were displayed at different time points after CCl4 injection (n = 4-7). *P<0.05, **P<0.01, ***P<0.001. (B) F4/80 and MPO Immunochemistry were performed in liver sections of WT mice and DJ-1 KO mice after CCl4 injection for 36h and 48h (400× magnification). (C) Cleaved-caspase 3 in liver sections from WT mice and DJ-1 KO mice after CCl4 injection for 36h was shown (n = 3) (200× magnification). Quantification of the positive areas was also performed (n = 3). **P<0.01. (D) mRNA levels of IL-6, TNF-α cytokines were determined by qPCR 36h post CCl4 injection. **P<0.01, ***P<0.001. (E) Immunochemistry for PCNA and Ki67 were performed after CCl4 injection for 36h and 48h respectively (200× magnification). Bar graphs show quantification of them (n = 5). **P<0.01, ***P<0.001. (F) ROS production was visualized by DCFH-DA probe in frozen liver sections from WT mice and DJ-1 KO mice 24h after CCl4 treatment (200× magnification). (G) MDA content in liver tissue homogenates was evaluated between WT mice and DJ-1 KO mice 24h after CCl4 injection. *P<0.05. (H) Gene expression of CYP2E1 in two groups was displayed 24h and 36h after CCl4 injection.