Skip to main content
. 2016 Sep 15;12(10):1225–1235. doi: 10.7150/ijbs.15154

Figure 4.

Figure 4

DJ-1 deletion suppresses ROS production and lipid peroxidation in CCl4-induced chronic liver injury. (A) Serum ALT and AST levels were determined in WT mice and DJ-1 KO mice after CCl4 injection for 8 weeks (n = 6-9). *P<0.05, **P<0.01. (B) Cleaved-caspase 3 in liver sections from WT mice and DJ-1 KO mice after CCl4 injection for 8 weeks was shown (n = 3) (200× magnification). Quantification of the positive areas was also performed (n = 3). *P<0.05. (C) Immunochemistry for MPO and CD11b were performed in liver sections of WT mice and DJ-1 KO mice after CCl4 injection for 8 weeks (400× magnification). Bar graphs show quantification of them, *P<0.05. (D) Gene expression of cytokines IL-6, TNF-α in the whole liver tissues was analyzed by qPCR after CCl4 injection for 8 weeks. *P<0.05. RFC: relative fold change. (E) ROS production was visualized in frozen liver sections and (F) MDA content was evaluated in liver tissue homogenates from WT mice and DJ-1 KO mice treated with CCl4 for 4 weeks (200× magnification). (G) Gene expression of CYP2E1 in two groups was displayed after 8 weeks CCl4 injection. (H) ROS production in isolated Kupffer cells, hepatocytes, HSCs was shown with mice treated with CCl4 for 4 weeks (100× magnification). (I) mRNA levels of HMOX-1 in cultured Kupffer cells, hepatocytes, HSCs were analyzed by qPCR with mice treated with CCl4 for 4 weeks. *P<0.05. RFC: relative fold change.