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. 2016 Mar 11;14(9):1838–1851. doi: 10.1111/pbi.12544

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© 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Quantitative real‐time PCR analyses of salt tolerance‐related marker genes. Three‐week‐old seedlings treated with 150 mm NaCl for 12 h or 0 h (Control) were harvested for total RNA extraction, transverse transcription and real‐time PCR analyses. WT, wild type; Z33 and Z38, independent transgenic lines. Values are means ± SD of three biological replicates from the WT or the transgenic lines. Asterisks indicate statistically significant difference in comparison with the WT (Student's t‐test, *, P < 0.05). The TIGR loci of these genes are as follows: NHX1 (Os07g0600900); SOS1 (Os12g0641100); P5CS (Os05g0455500); SPY‐like (Os08g0559300); DREB1A (Os09g35030); DREB1F (Os01g73770); DREB2A (Os01g07120).