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. 2004 Sep;78(17):9144–9153. doi: 10.1128/JVI.78.17.9144-9153.2004

FIG. 3.

FIG. 3.

(A) Intracellular distribution of PB1 in the absence or presence of PB2 and PA. 293T cells expressing PB1 (lanes 1 and 2) or PB1, PB2, and PA (lanes 3 and 4) were fractionated into cytoplasmic (C) and nuclear (N) fractions, and equivalent amounts were subjected to SDS-PAGE and Western blotting with a polyclonal anti-PB1 antibody. (B) Expression and binding to PB1 of mutant PA proteins. Extracts from 293T cells expressing PA (wild type [WT] or the indicated deletion mutants) and TAP-tagged PB1 (37) were analyzed by SDS-PAGE and Western blotting with a polyclonal anti-PA antibody before (left panel) and after (right panel) purification. Note the cross-reaction of PB1-TAP in the left panel due to the presence of the TAP tag. Sizes (in kilodaltons) are indicated on the left.