Figure 1. Starvation resistance and life span of animals with altered OA signalling.

Tβh^nM18 males (A) and females (B) as well as their matching controls were analysed regarding their starvation resistance. Ectopic activation of OA release by the expression of the temperature-sensitive cation channel TrpA1 (Tβh-Gal4 × UAS-TrpA1, blue) was compared with that of matching genetic controls (Tβh-Gal4 × w¹¹¹⁸ and w¹¹¹⁸ × UAS-TrpA1, black) (C). All flies were held at the permissive temperature (30 °C), and at least five independent biological replicates were analysed. Cohorts of male (D) and female (E) control (black) and Tβh^nM18 (red) adult flies were scored for survival. Cohorts of more than 100 animals were used for all survival experiments. Statistical analyses were performed using the log-rank test. *p < 0.05, **p < 0.01 and ***p < 0.001 for all figures.