Figure 2. Determination of triglyceride and glycogen levels in flies with altered OA signalling.

Triglyceride contents of Tβh^nM18 male and female flies challenged with normal (A, left) and high-fat diets (A, right). BODIPY staining of Tβh^nM18 female flies (B, left Tβh^nM18, right matching control). Triglyceride contents of flies in which the temperature-sensitive TrpA1 channel (UAS-TrpA1) was expressed in OA-producing cells (driven by Tβh-Gal4) and the corresponding genetic controls (Tβh-Gal4 × w¹¹¹⁸ and w¹¹¹⁸ × UAS-TrpA1) maintained at the restrictive (19 °C, black) or permissive (30 °C, blue) temperature (C). Glycogen contents of male and female Tβh^nM18 and matching controls are presented (D). Triglyceride contents of Tdc2^RO54 male and female flies (green) as well as their matching controls (black, E). Statistics, see Fig. 1.