Figure 3. Food intake and metabolic rate of Tβh-deficient animals.

Food intake of male and female flies was quantified using the CAFE assay (A). Tβh^nM18 (A, red) and control (A, black), Tdc2^RO54 (B, green) and control (B, black); the means of four biological replicates ± SEM are shown. Metabolic rates of cohorts of three adult flies were quantified according to Yatsenko et al.²⁷, using Tβh^nM18 and their matching controls (C) or Tdc2^RO54 and their matching controls (D). At least five biological replicates were analysed. Shown are mean values ± SEM. Statistics, see Fig. 1.