Figure 4. Movement activity and sleep in flies with altered OA signalling.

Overall movement activity was analysed using the Drosophila activity monitoring system (A,C–F). Twenty-four-hour periods of activity were scored for Tβh^nM18 (A, red) adults and matching controls (A, black). Climbing performance was tested using a negative geotaxis assay using Tβh^nM18 and control flies of both sexes. Animals were scored after fixed time points based on their height on a vertical plane (B). Movement activities of animals with ectopically induced release of OA (blue, Tβh-Gal4 × UAS-TrpA1) were compared with those of both matching genetic controls (grey, Tβh-Gal4 × w¹¹¹⁸ and w¹¹¹⁸ × UAS-TrpA1) using the Drosophila activity monitoring system. Flies were analysed at 30 °C (C). Total sleep time was assessed from the data obtained with the Drosophila activity monitor (D). For all experiments, mean values of 5–10 independent experiments ± SEM are shown. Sleep activity in representative 48 h intervals is shown for male (E) and female flies (F). *p < 0.05, **p < 0.01 and ***p < 0.001.