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. 2016 Oct 19;6:35656. doi: 10.1038/srep35656

Figure 4. YFP-MAPPER and FDM identify ER-PM junctions in TIRFM.

Figure 4

(a) Top, left: TIRFM micrographs of a non-store depleted RBL-2H3 cell, co-expressing YFP-MAPPER (green, left) and cytosolic mCherry (cyan, right). Below: Magnified details of area indicated in top panel including a color overlay (merge). Right: Intensity line-scans of YFP-MAPPER fluorescence (green) and FDM intensities (blue) as indicated by the dotted line in detail image. (b) Top, left: TIRFM micrographs of a store depleted RBL-2H3 cell, co-expressing YFP-MAPPER (green, left) and cytosolic mCherry (cyan, right). Below: Magnified details of area indicated in top panel, including a color overlay (merge). Right: Intensity line-scans of YFP-MAPPER fluorescence (green) and FDM intensities (blue) as indicated by the dotted line in detail image. Bars = 5 μm.