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. 2016 Oct 19;6:35387. doi: 10.1038/srep35387

Figure 4. Neointima formation 4 weeks after dual vascular injury in NSG mice reconstituted with or without human PBMCs.

Figure 4

Representative sections of carotid and femoral arteries from dual injured and sham-operated mice stained with Verhoeff-van Gieson. (A) Sham- operated control carotid artery, (B) Sham-operated control femoral artery, (C) Carotid artery after wire injury in a non-reconstituted NSG mouse, (D) Femoral artery after cuff injury in a non-reconstituted NSG mouse, (E) Carotid artery after wire injury in a human PBMC-reconstituted NSG mouse, (F) Femoral artery after cuff injury in a human PBMC-reconstituted NSG mouse. Squares indicate areas shown at higher-power magnification of the respective cross-sections (C.1, D.1, E.1, F.1). Neointima is indicated as tissue in between arrowheads. Neointima formation in the carotid and femoral arteries of dual injured NSG mice reconstituted with (n = 6) and without (n = 9) human PBMCs was expressed as % intimal expansion (G) and intima/media ratio (H). *P < 0.05, **P < 0.01. Data are expressed as mean ± SEM. Abbreviations: m: media; ni: neointima; PBMCs: human peripheral blood mononuclear cells. Original magnification (A–F): 200x.