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. 2016 Oct 19;6:35387. doi: 10.1038/srep35387

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Sham-operated (non-injured) and wire-injured carotid arteries stained for αSMA/calponin (A) and αSMA/SM22α (B). Sham-operated and cuff-injured femoral arteries stained for αSMA/calponin (C) and αSMA/SM22α (D). As negative control, cuff-injured femoral arteries were incubated according the protocol but with omitting primary antibody incubation (E). Insets and single stainings show high power magnifications of the boxed regions. Acquired photomicrographs were converted into pseudocolored images and merged using ImageJ 1.47v (http://imagej.nih.gov/ij). Abbreviations: a: adeventitia; m: media; ni: neointima.