Figure 6.
Presence of human PBMC-derived cells within injured carotid (A–D) and femoral (E–H) arteries in NSG mice 4 weeks after reconstitution with 5 × 106 human PBMCs. Sections were immunofluorescently stained with αSMA (in red) and specific antibodies (in green) recognizing human CD45 (A,E), human CD4 (B,F), human CD8 (C,G) and human CD68 (D,H). Representative images of arterial staining from 3 different mice per group. Specificity controls for the antibodies used are shown in Figs S6 and S7A. Nuclei were stained with DAPI (in blue). Dotted line indicates the internal elastic lamina. Arrows indicate positively stained human leukocytes. Original magnification: 400x. Confocal microscopy ortho images hCD45/αSMA (I) or hCD8/αSMA (J) on wire-injured femoral arteries. Abbreviations: a: adventitia; m: media; ni: neointima.