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. 2016 Oct 19;6:35647. doi: 10.1038/srep35647

Figure 3. Lineage labelling of renal precursors.

Figure 3

(A) Dorsal views of flat-mounted embryos that are double stained for slc4a4 (purple) and myod1/smyhc1 (red) are shown at the indicated stages. (B) Panels show uncaging of a lineage tracer in the intermediate mesoderm lateral to somite 8 at the 12-somite stage and tracking of these cells posteriorly (white arrows) up to the 24 hpf stage (upper panels show live brightfield images, middle panels show live fluorescent images). Lower panels show lateral views of the trunk from the same fate-mapped embryo double stained for the lineage tracer (‘anti-flu’; purple) and slc12a1 (red).