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. 2016 Oct 19;6:35531. doi: 10.1038/srep35531

Figure 2. SRA inhibits FFA oxidation by repressing ATGL expression in hepatocytes.

Figure 2

(a) SRA and ATGL mRNA levels in primary hepatocytes isolated from SRAKO (KO, n = 8) or WT (n = 8) littermates (7–8 weeks of age, chow diet) were analyzed by RT-qPCR. Protein expression of ATGL and SRAP was assessed by immunoblotting. (b) ATGL mediated ketone body production was measured in hepatocytes indicated in (a). (c) Endogenous SRA in Hepa1-6 cells was knocked down by lentiviral infection with shRNA against SRA (shSRA) or scrambled shRNA as control (shControl). Subsequent assays of mRNA and protein expression were performed 72 h after infection. (d) ATGL mediated ketone body production was measured in Hepa1-6 cells with shControl and shSRA knocked down at the same condition as (c). (e) Hepa1-6 cells were transfected with pSCT (Control), pSCT-SRA (SRA) or pSCT-SRAP-SDM1/7 (SRAP) expression vectors, and subsequent assays of RNA and protein expressions were performed 60 h after transfection. (f) ATGL-mediated ketone body production was measured in Hepa1-6 cells described in (e). The mRNA levels of genes were normalized to 36B4 expression. The data are presented as the mean ± SE, *p < 0.05.