FIG. 2.

Tat increases vGPCR-mediated NF-κB and NF-AT activation. Shown are results of ELISA-based DNA binding assays for NF-κB (A and C) and NF-AT (B and D) carried out as described in Materials and Methods on transfected GR3 cells (A and B) or on lysates of nude mouse tumors (C and D). The black bars show binding to deoxyoligonucleotides containing consensus binding sites immobilized on plastic and analyzed as described in Materials and Methods. The binding was competed with excess wild-type deoxyoligonucleotides (open bars) or deoxyoligonucleotides containing mutated binding sites (checkered bars). OD, optical density.