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. 2016 Sep 19;35(20):2223–2237. doi: 10.15252/embj.201693991

Figure 3. Snap29 depletion affects mitotic progression.

Figure 3

  1. Selected frames of time‐lapse imaging of control S2 cells or cells treated for 96 h with dsRNA against the central portion of Snap29. Note that depleted cells do not display a recognizable metaphase plate, or fail to segregate all chromosomes correctly, or form tripolar spindles or micronuclei (arrowheads; arrowheads in control point to cells dividing normally).
  2. Western blotting of extract from control cells and cells depleted of Snap29 (dsSnap29) relative to the experiment in (A).
  3. Quantification of the mitotic phenotype of control and dsSnap29‐depleted cells, based on time‐lapse imaging of an average of > 28 individual cells per sample. The mean with standard error of the mean (SEM) is shown, and P‐value is determined by two‐tailed t‐test considering all defects together. *P ≤ 0.05.
  4. Selected frames of time‐lapse imaging of control U2OS cells and U2OS cells treated for the indicated time. Note that the depleted cell at 48–66 h display altered metaphase plate and chromosome segregation (arrowheads).
  5. Western blot analysis of extracts relative to the experiments in (D).
  6. Quantification of the time from prophase to entry in anaphase based on analysis of > 20 individual cells/sample by time‐lapse microscopy. The mean with standard error of the mean (SEM) is shown, and P‐values are determined by Kruskal–Wallis test with Dunn's multiple comparison analysis. *P ≤ 0.05. OE: overexpression.