Table 1. Data collection and refinement statistics for FlgEcc32 and FlgEcj79 crystals.
| FlgEcc32 |
FlgEcj79 |
||||
|---|---|---|---|---|---|
| Native | SeMet peak | Native | SeMet peak | SeMet edge | |
| Data collection | |||||
| Space group | P21 | P21 | P21 | P21 | P21 |
| Cell dimensions (Å,°) | |||||
| a | 54.54 | 54.84 | 75.46 | 75.45 | 75.45 |
| b | 61.30 | 61.50 | 173.54 | 173.76 | 173.76 |
| c | 102.86 | 103.11 | 147.09 | 147.69 | 147.69 |
| β | 91.37 | 91.70 | 102.66 | 102.98 | 102.98 |
| Wavelength (Å) | 0.9 | 0.9791 | 1.0 | 0.9791 | 0.9794 |
| Resolution (Å) | 50.0–1.84 (1.87–1.84) | 50.0–2.00 (2.03–2.00) | 41.0–2.45 (2.49–2.45) | 37.69–2.60 (2.74–2.60) | 37.71–2.60 (2.74–2.60) |
| Rmerge | 9.4 (30.4) | 8.8 (20.1) | 12.6 (91.4) | 13.0 (45.6) | 13.7 (52.1) |
| Mean <I/σ (I)> | 13.6 (2.2) | 32.3 (10.5) | 24.2 (2.7) | 8.0 (3.0) | 7.4 (2.7) |
| Completeness (%) | 97.6 (92.6) | 100 (99.3) | 99.5 (99.0) | 99.9 (99.9) | 100 (100) |
| Redundancy | 3.0 (2.3) | 5.1 (5.0) | 11.2 (10.9) | 3.8 (3.7) | 3.8 (3.8) |
| Refinement | |||||
| Resolution (Å) | 19.8–1.84 | 25.0–2.45 | |||
| No. reflections | 59519 | 133379 | |||
| Rwork/Rfree | 17.6/22.2 | 20.7/25.4 | |||
| No. atoms | |||||
| Protein | 4402 | 21712 | |||
| Water | 1114 | 828 | |||
| B-factors | |||||
| Protein | 14.4 | 60.7 | |||
| Water | 25.1 | 43.1 | |||
| R.m.s deviations | |||||
| Bond length (Å) | 0.005 | 0.005 | |||
| Bond angle (°) | 0.93 | 0.91 | |||