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Influence of the position of the splGFP11 tag on the SplitGFP signal. The highest expressed NOD2 construct, ND1 was tagged N‐ or C‐ terminally with different spacer elements with the splGFP11 tag. The resulting SplitGFP response was measured in the BioLector. The BioLector sensitivity was slightly increased compared to the data shown in Figure 3 to accommodate the lower GFP signal of the C‐terminal tagged constructs.
