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. 2004 Sep;78(17):9164–9173. doi: 10.1128/JVI.78.17.9164-9173.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 1. — CD3/CD28 costimulation induces high levels of activation marker expression in CD4⁺ T cells. CD4⁺ T cells were isolated from PBMC of three donors and then stimulated with CD3/CD28 antibodies or PHA for 0, 24, 48, or 72 h. At each time point, cells were stained with fluorescently conjugated monoclonal antibodies directed against CD4 and the activation markers CD38 and CD69. Samples were acquired with a FACSCalibur system (Becton Dickinson), and the resulting data were analyzed using CellQuest software (Becton Dickinson). (A) Representative flow cytometric results from one donor. The number in the upper-right hand corner of each FACS plot represents the percentage of CD38⁺ CD69⁺-coexpressing cells. (B) The percentage of CD38⁺ CD69⁺-coexpressing cells detected in each donor was averaged and plotted as a line graph, with error bars representing the variation among donors.

FIG. 1. — CD3/CD28 costimulation induces high levels of activation marker expression in CD4⁺ T cells. CD4⁺ T cells were isolated from PBMC of three donors and then stimulated with CD3/CD28 antibodies or PHA for 0, 24, 48, or 72 h. At each time point, cells were stained with fluorescently conjugated monoclonal antibodies directed against CD4 and the activation markers CD38 and CD69. Samples were acquired with a FACSCalibur system (Becton Dickinson), and the resulting data were analyzed using CellQuest software (Becton Dickinson). (A) Representative flow cytometric results from one donor. The number in the upper-right hand corner of each FACS plot represents the percentage of CD38⁺ CD69⁺-coexpressing cells. (B) The percentage of CD38⁺ CD69⁺-coexpressing cells detected in each donor was averaged and plotted as a line graph, with error bars representing the variation among donors.