Figure 4. Activity profile of the reconstituted cellulosomes for crystalline cellulose and delignified rice straw.

The enzymatic activities for Avicel and delignified rice straw of the cellulosome complexes reconstituted from 3 (circles), 12 (triangles), 30 (diamonds), and 40 (squares) components were determined by measuring the amount of reducing sugars released from 0.5% substrate at 55 °C for 24 h. Cellulosome complexes were assembled by mixing CipA at various concentrations with the enzyme mix at a fixed concentration (0.02 μg/μl). The activities of enzyme-saturated complexes assembled at a molar ratio of CipA/enzyme = 1/9 (cohesin/dockerin = 1/1) are shown by filled symbols. The enzymatic activity for Avicel of the cellulosome complexes reconstituted from 3 components was measured previously⁹. Data are presented as the means from three independent experiments, and ±SE values are shown.