Table 1. Enzymatic activities of reconstituted cellulosomes for crystalline cellulose and delignified rice straw.
CipA/enzymeb | Specific activity (U/mg)a |
|||
---|---|---|---|---|
Avicel | Rice straw | |||
0 | 0.058 ± 0.0061 | (1.0)c | 0.090 ± 0.010 | (1.0)c |
1/9 | 0.17 ± 0.024 | (2.9)c | 0.21 ± 0.030 | (2.3)c |
1/2.25 | 0.15 ± 0.016 | (2.7)c | 0.27 ± 0.015 | (3.0)c |
aCellulosome complexes were assembled by mixing CipA at various concentrations and the 40 enzyme mix at a fixed concentration. Assays were performed at different concentrations of enzyme to determine if the amount of product increased in proportion to the amount of enzyme. Data are presented as the means from three independent experiments, and ±SE are shown.
bThe values of CipA/enzyme indicate the molar ratio of the scaffoldin protein relative to the enzyme mix.
cThe relative activities of the cellulosome complexes to the unassembled enzyme mixture are indicated in parentheses.