Table 2.
Short‐term modulations in photosynthetic O2 and inorganic carbon (Ci) fluxes of Emiliania huxleyi in response to low dissolved inorganic carbon (DIC) and high pH (LDICHpH), as well as carbonation (HDICHpH), acidification (LDICLpH) and the combination (HDICLpH)
Acclimation | PFD | Carbonate chemistry | Phot (μmol mg−1 h−1) | Resp(μmol mg−1 h−1) | CO2uptPS(μmol mg−1 h−1) | HCO3 −uptPS(μmol mg−1 h−1) |
|
|
---|---|---|---|---|---|---|---|---|
2N LL | 180 | LDICHpH | 217 ± 17 | 75 ± 7 | 51 ± 20 | 147 ± 15 | 0.26 ± 0.09 | |
HDICHpH | 236 ± 34 | 63 ± 9 | 58 ± 19 | 156 ± 43 | 0.28 ± 0.11 | |||
LDICLpH | 271 ± 63 | 26 ± 17 | 221 ± 49 | 25 ± 29 | 0.90 ± 0.11 | |||
HDICLpH | 345 ± 22 | 42 ± 8 | 303 ± 28 | 11 ± 9 | 0.96 ± 0.03 | |||
700 | LDICHpH | 327 ± 5 | 59 ± 10 | 59 ± 1 | 239 ± 1 | 0.20 ± 0.08 | ||
HDICHpH | 355 ± 19 | 57 ± 20 | 78 ± 1 | 245 ± 1 | 0.24 ± 0.15 | |||
LDICLpH | 365 ± 69 | 27 ± 14 | 226 ± 1 | 64 ± 1 | 0.76 ± 0.23 | |||
HDICLpH | 464 ± 48 | 46 ± 7 | 393 ± 1 | 29 ± 1 | 0.93 ± 0.06 | |||
2N HL | 180 | LDICHpH | 181 ± 14 | 72 ± 21 | 23 ± 39 | 141 ± 40 | 0.14 ± 0.25 | |
HDICHpH | 141 ± 2 | 65 ± 8 | 32 ± 17 | 96 ± 15 | 0.25 ± 0.13 | |||
LDICLpH | nd | nd | nd | nd | nd | |||
HDICLpH | 124 ± 28 | 67 ± 3 | 77 ± 36 | 35 ± 16 | 0.67 ± 0.17 | |||
700 | LDICHpH | 360 ± 30 | 80 ± 11 | 56 ± 77 | 271 ± 68 | 0.17 ± 0.22 | ||
HDICHpH | 307 ± 40 | 64 ± 17 | 55 ± 18 | 224 ± 23 | 0.19 ± 0.05 | |||
LDICLpH | nd | nd | nd | nd | nd | |||
HDICLpH | 225 ± 32 | 60 ± 4 | 93 ± 46 | 129 ± 18 | 0.40 ± 0.17 | |||
1N LL | 180 | LDICHpH | 147 ± 22 | 38 ± 8 | 1 ± 8 | 131 ± 14 | 0.00 ± 0.06 | |
HDICHpH | 173 ± 69 | 29 ± 7 | 28 ± 26 | 129 ± 39 | 0.15 ± 0.10 | |||
LDICLpH | 202 ± 29 | 56 ± 12 | 58 ± 16 | 126 ± 19 | 0.31 ± 0.07 | |||
HDICLpH | 185 ± 79 | 29 ± 3 | 55 ± 83 | 113 ± 17 | 0.24 ± 0.33 | |||
700 | LDICHpH | 223 ± 32 | 35 ± 4 | −6 ± 8 | 207 ± 23 | −0.03 ± 0.04 | ||
HDICHpH | 240 ± 56 | 29 ± 5 | 7 ± 21 | 210 ± 41 | 0.02 ± 0.09 | |||
LDICLpH | 286 ± 23 | 48 ± 11 | 26 ± 17 | 225 ± 25 | 0.11 ± 0.07 | |||
HDICLpH | 273 ± 23 | 32 ± 4 | 38 ± 52 | 210 ± 37 | 0.14 ± 0.19 | |||
1N HL | 180 | LDICHpH | 119 ± 13 | 69 ± 4 | −33 ± 7 | 141 ± 11 | −0.31 ± 0.09 | |
HDICHpH | 113 ± 36 | 54 ± 8 | −12 ± 26 | 114 ± 6 | −0.18 ± 0.30 | |||
LDICLpH | 136 ± 19 | 67 ± 19 | 0 ± 17 | 124 ± 24 | 0.00 ± 0.14 | |||
HDICLpH | 148 ± 34 | 79 ± 20 | −20 ± 17 | 154 ± 44 | −0.14 ± 0.10 | |||
700 | LDICHpH | 246 ± 13 | 68 ± 8 | −38 ± 10 | 261 ± 6 | −0.17 ± 0.05 | ||
HDICHpH | 196 ± 33 | 55 ± 9 | −29 ± 24 | 207 ± 24 | −0.18 ± 0.15 | |||
LDICLpH | 236 ± 25 | 56 ± 8 | −5 ± 8 | 208 ± 33 | −0.02 ± 0.03 | |||
HDICLpH | 274 ± 68 | 75 ± 9 | −23 ± 2 | 272 ± 60 | −0.10 ± 0.03 |
Chla‐ normalized photosynthetic net O2 evolution (Phot) and respiration (Resp), photosynthetic net CO2 uptake (CO2uptPS), photosynthetic HCO3 − uptake (HCO3 −uptPS) and the fraction of overall photosynthetic net Ci uptake that is covered by net CO2 uptake () were measured at low and high photon flux densities (PFD; 180 vs 700 μmol photons m−2 s−1; n = 3; ± SD).
2N LL/HL, diploid life‐cycle stage acclimated to low light/high light; 1N LL/HL, haploid life‐cycle stage acclimated to low/high light; LDICHpH, low DIC/high pH; HDICHpH, high DIC/high pH; LDICLpH, low DIC/low pH; HDICLpH, high DIC/low pH; nd, not determined.