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A cell culture supernatant from the same cell line as that used for growing virus has no TNF-α- or IFN-γ-inducing activity. Whole-blood cultures were stimulated with PPVO (virus titer, 104.6/ml), virus-free cell culture supernatant (CCS), and RPMI 1640 medium as a negative control for 4 h for TNF-α induction and for 2 days in the presence of ConA (1 μg/ml) for IFN-γ induction.
