Figure 1.
Expression of yNluc and yNlucPEST gene reporters in yeast. (A) Schematic representation of yNanoluc and yNlucPEST yeast codon‐optimized gene reporter cassettes designed for PCR amplification and integration into the yeast genome: a dominant G418 selection marker is provided by kanMX; for the purposes of this study, the minimal CYC1 promoter (PCYC1) fused to a heat shock element (HSE) drives the expression of each reporter variant. (B) Bioluminescence (bioluminescence light units, BLU) in cells carrying vector control (VC) or expressing yNluc or yNlucPEST; error bars represent SD (n = 3). (C) Turnover of yNluc and yNlucPEST bioluminescence following arrest of translation by cycloheximide (CHX) or lactimidomycin (LTM) addition; error bars represent SD (n ≥ 3)