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. 2016 Oct 19;6:35611. doi: 10.1038/srep35611

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Copyright © 2016, The Author(s)

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(A) Average expression stability values of remaining control genes. M was excluded in a stepwise manner until the two most stable genes remained: hsa-miR-25-3p and hsa-miR-106-5p. (B) Determination of the optimal number of control genes for normalization. The number of reference genes is considered optimal when the V value reached the lowest, at which point it is unnecessary to include additional genes in the normalization strategy.